Wheat protein isolates and processes for producing

ABSTRACT

Isolated wheat proteins that are substantially free of additives, processing aids, added acids, buffers, reagents, or added enzymes and compositions formed from the isolated wheat proteins are described. Methods of forming compositions which include isolated wheat proteins that are substantially free of additives, processing aids, added acids, buffers, reagents, or added enzymes are also described.

CROSS REFERENCE TO RELATED APPLICATIONS

This application claims priority to U.S. Provisional Patent ApplicationNo. 61/501,306, filed Jun. 27, 2011, the contents of the entirety whichis incorporated by this reference.

TECHNICAL FIELD

Various embodiments of the present disclosure relate to compositionscomprising wheat proteins that are produced without the use of addedacids or reducing agents, such as sulfites. Other embodiments relate toprocesses of isolating wheat proteins naturally, without any additives,processing aids, added acids, reagents, buffers, or added enzymes.

BACKGROUND

The development and production of wheat proteins isolates has continued.US Patent Application 2008/0254200 published about Oct. 16, 2008describes the production of wheat protein isolates that aresubstantially free of sulfites. While the invention described in USPatent Application 2008/0254200 successfully produced a wheat proteinisolate without using sulfites, such publication still used an addedacid, such as lactic acid.

Wheat protein isolates are used in the food industry as an ingredient toalter texture and enhance taste and appearance in food products. Wheatprotein isolates may add certain benefits to food products, includingreplacing sugar or carbohydrate functionalities in baked or processedfoods; building structure or improving crumb texture in baked goods;improving freeze-thaw performance with improved texture and mouthfeel;replacing sugar as a binder in bars and coatings for cereals; increasingprotein levels in foods without sacrifice of taste and texture;improving dough rheology, proofing times, and sheeting performance;reducing fat in food products; generating foam and other types ofcontrolled air entrapment; and improving taste and texture in wholegrain applications.

While US Patent Application 2008/0254200 successfully produced a wheatprotein isolate without a reducing agent, a need exists for theproduction of wheat protein isolates that do not have any additives,processing aids, added acids, buffers, reagents, or added enzymes andthat have a clean ingredient label.

SUMMARY OF THE INVENTION

In each of its various embodiments, the present invention fulfills theseneeds and discloses methods for producing wheat proteins withoutadditives, processing aids, added acids, buffers, reagents, or addedenzymes. In another embodiment, the present invention is directedtowards a naturally produced wheat protein isolate that has uniqueproperties such as rheology, water adsorption, taste, film forming, andbinding.

In one embodiment, a composition comprises an isolated wheat protein.The composition is substantially free of additives, processing aids,reagents, added acids, buffers, and added enzymes, and has less than 10%moisture by weight.

In another embodiment, a process for producing an isolated wheat proteincomprises mixing water and wheat gluten to form a gluten slurry andagitating the gluten slurry at a temperature between 15° C. and 55° C.for a time sufficient to produce an isolated wheat protein. An acid andbuffer are not added to the gluten slurry during the process.

BRIEF DESCRIPTION OF THE DRAWINGS

FIG. 1 shows a mixograph of one embodiment of a wheat protein isolateproduced according to the present invention.

FIG. 2 shows a mixograph of one embodiment of a wheat protein isolateproduced according to the present invention.

FIG. 3 shows a mixograph of one embodiment of a wheat protein isolateproduced according to the present invention.

FIG. 4 is an alveograph of an embodiment of a wheat protein isolateproduced according to the present invention.

DETAILED DESCRIPTION OF THE INVENTION

Various embodiments of the present disclosure relate to processes forproducing isolated wheat proteins and the compositions producedtherefrom. The wheat protein isolates of the present invention arenatural in that they don't have additives, processing aids, added acids,buffers, reagents, or added enzymes and have a clean ingredient label.

Other than the operating examples or where otherwise indicated, allnumbers expressing quantities of ingredients, processing conditions, andthe like used in the specification and claims are to be understood asbeing modified in all instances by the term “about”. Unless indicated tothe contrary, the numerical parameters set forth in the followingspecification and claims are approximations that may vary depending uponthe desired properties sought to be obtained. At the very least, and notas an attempt to limit the application of the doctrine of equivalents tothe scope of the claims, each numerical parameter should at least beconstrued in light of the number of reported significant digits and byapplying ordinary rounding techniques.

Any numerical values disclosed herein may inherently contain certainerrors, such as, equipment and/or operator error, necessarily resultingfrom the standard deviation found in their respective testingmeasurements.

Also, it should be understood that any numerical range recited herein isintended to include all sub-ranges subsumed therein. For example, arange of “1 to 10” is intended to include all sub-ranges between (andincluding) the recited minimum value of 1 and the recited maximum valueof 10, that is, having a minimum value equal to or greater than 1 and amaximum value of less than or equal to 10.

Any patent, publication, or other disclosure material, in whole or inpart, that is said to be incorporated by reference herein isincorporated herein only to the extent that the incorporated materialdoes not conflict with existing definitions, statements, or otherdisclosure material set forth herein. As such, and to the extentnecessary, the disclosure set forth herein supersedes any conflictingmaterial incorporated by reference. Any material, or portion thereof,that is said to be incorporated by reference, but which conflicts withexisting definitions, statements, or other disclosure material set forthherein will only be incorporated to the extent that no conflict arisesbetween that incorporated material and the existing disclosure material.

The present disclosure describes several different features and aspectsof the various exemplary embodiments. It is understood, however, thatthe present disclosure embraces numerous alternative embodiments, whichmay be accomplished by combining any of the different features, aspects,and embodiments described herein in any combination that one of ordinaryskill in the art would find useful.

According to certain embodiments, the present disclosure provides for acomposition comprising an isolated wheat protein that is producednaturally in that it does not include additives, processing aids, addedacids, buffers, reagents, or added enzymes. In one embodiment, theisolated wheat protein may comprise at least 60% protein by weight, asdetermined by measuring nitrogen content and multiplying by 5.7. Inanother embodiment, the protein content may be determined by measuringnitrogen content and multiplying by 6.25. As used herein, the term“substantially free” when used in reference to additive, processing aid,added acid, buffer, reagent, or added enzyme concentrations, includesthe additive, the processing aid, the added acid, buffer, the reagent,or the added enzyme concentration that is below the measurable limitusing standard analytical procedures. According to certain embodiments,the isolated wheat protein may have an additive, processing aid, addedacid, buffer, reagent, or added enzyme concentration of less than 0.001%by weight (i.e., less than 10 ppm). According to other embodiments, theisolated wheat protein may have an additive, processing aid, added acid,buffer, reagent, or added enzyme concentration of 0% by weight.

There has been a growing desire for products and food compositions thatare all natural and/or organic (as defined by various regulatingagencies). Certain regulatory agencies may limit or regulate howcompositions containing additives, processing aids, added acids,buffers, reagents, or added enzymes may be described. For example, incertain markets, the presence of additives, processing aids, addedacids, buffers, reagents, or added enzymes in a product or foodcomposition may preclude the use of certain monikers, descriptors, orindicia. For example, in certain markets, the presence of additives,processing aids, added acids, buffers, reagents, or added enzymes in aproduct or food composition may prevent the use of descriptors, such as,for example, “organic” or “natural” when describing the product or foodcomposition. Thus, modified wheat proteins that are produced byrecognized commercial processes may not be described as “natural” or“organic” due to these restrictions. However, the compositions of thepresent disclosure comprising isolated wheat proteins that aresubstantially free of additives, processing aids, added acids, buffers,reagents, or added enzymes will not be subject to these regulations.

The isolated wheat proteins of the present disclosure may have improvedproperties and characteristics compared to wheat protein isolates thatare not produced naturally. For example, the isolated wheat proteins ofvarious embodiments described herein may have improved properties suchas rheology, water absorption, taste, film forming, or binding.

As described herein, the isolated wheat proteins of the presentdisclosure may be produced from wheat gluten, vital wheat gluten, wheatprotein isolates, or wheat protein concentrates. Wheat protein isolatesmay have a protein content of at least 75% by weight, whereas wheatprotein concentrates may have a protein content of at least 60% byweight. Thus, the isolated wheat proteins of the present disclosure mayhave a protein content of at least 60% by weight. According to otherembodiments, the isolated wheat proteins of the present disclosure mayhave a protein content of at least 75% by weight. According to stillother embodiments, the isolated wheat proteins of the present disclosuremay have a protein content of at least 80% by weight. In certainembodiments, the isolated wheat proteins of the present disclosure mayhave a maximum protein content of 99% by weight. The protein content ofthe isolated wheat proteins of the present disclosure may be measured byany method known in the art. For example, one method of measuring theprotein content in a food composition is the Kjeldahl method whichinvolves measuring the nitrogen content in the modified wheat proteinisolates (for example by a chemical degradation) and multiplying by 5.7(corresponding to the nitrogen content of wheat proteins). In otherembodiments, the protein content may be calculated by multiplying thenitrogen content by 6.25. Other methods for measuring the nitrogencontent in the isolated wheat proteins, such as combustion nitrogenanalysis (the “Dumas method”), may also be used.

The present disclosure also provides for food compositions that comprisethe isolated wheat proteins that are substantially free of additives,processing aids, added acids, buffers, reagents, or added enzymesaccording to any the various embodiments disclosed herein. Foodcompositions may include, but are not limited to, baked goods, processedfoods, whole grain foods, food bars, cereals, granolas, doughs andbatters, cakes, tortillas, snacks, cookies, microwave bakery products,whips, fillings, frostings, frozen foods, wieners, sausages, meat loaf,meat patties, dressings, spreads, pet foods, fish feeds, shrimp feed, oranimal feeds.

According to other embodiments, the present disclosure providesprocesses for producing an isolated wheat protein that are substantiallyfree of additives, processing aids, added acids, buffers, reagents, oradded enzymes. According to one embodiment, the process for producing anisolated wheat protein may comprise mixing water and wheat gluten toform a gluten slurry, agitating the gluten slurry at a reactiontemperature for a time sufficient to produce an isolated wheat protein,wherein the isolated wheat protein is substantially free of additives,processing aids, added acids, buffers, reagents, or added enzymes.According to certain embodiments, the isolated wheat protein may have anadditive, processing aid, added acid, buffer, reagent, or added enzymeconcentration of less than 0.001% by weight (less than 10 ppm).According to other embodiments, the isolated wheat protein may have anadditive, processing aid, added acid, buffer, reagent, or added enzymeconcentration of 0% by weight. In another embodiment, a pH of the glutenslurry after adding the gluten to the water is above pH 4.5.

In certain embodiments, the reaction temperature may range from 15° C.to 52° C. In other embodiments, the temperature may range from 34° C. to45° C. In other embodiments, the temperature may range from 43° C. to47° C.

The gluten slurry may be formed by mixing water and wheat gluten, suchas a wet wheat gluten, to form the slurry. In certain embodiments, themixing may be performed at the reaction temperature or a temperaturehigher than the reaction temperature (such as the water temperature).The wheat gluten may be added to the water in such an amount to resultin a gluten slurry having a total solids content ranging from 6% to 22%on a dry basis. In another embodiment, the gluten slurry may have atotal solids content ranging from 11% to 18% on a dry basis. In anotherembodiment, the gluten slurry may have a total solids content rangingfrom 14% to 16.5% on a dry basis.

The gluten slurry may be agitated at the reaction temperature for a timesufficient to produce an the gluten slurry having a desired viscosity.For example, according to certain embodiments, the gluten slurry mayhave a Brookfield viscosity (as defined herein) ranging from 170 cps to360 cps. As used herein, “agitation” and “agitating” include stirringthe gluten slurry, mixing the gluten slurry, mixing the gluten slurrywith a high shear (e.g., using a high shear mixer), agitating the glutenslurry, and sonicating the gluten slurry or any combinations of theseagitation methods. In certain embodiments, agitating the gluten slurryis performed using a high shear, such as, for example, mixing at a highspeed. In other embodiments, agitating the gluten slurry is performedusing a standard mixing speed. As the gluten slurry is agitated over thereaction time, the viscosity of the slurry will decrease. In variousembodiments, as the gluten slurry viscosity decreases, the mixing speedmay also be decreased. For example, in one embodiment, after theviscosity of the gluten slurry has decreased to approximately 200 cps to220 cps, the speed of the agitator may be lowered, for example from ahigh speed to a medium or low speed.

In other embodiments, the gluten slurry may be agitated for a timesufficient to produce an isolated wheat protein having a Brookfieldviscosity ranging from 17 cps to 390 cps at room temperature. In certainembodiments, the gluten slurry may be agitated, for example, at a highspeed, for a time period ranging from 175 minutes to 380 minutes. Inanother embodiment, the time period may range from 190 minutes to 230minutes. In other embodiments, wherein a lower or more vigorousagitation is used, it will be understood that the agitation time will beadjusted accordingly. For example, in certain embodiments, where a lowerenergy agitation is used, the agitation time may be increased, whereasin embodiments where a higher energy agitation is used, the agitationtime may be shortened.

After agitating the gluten slurry to form the isolated wheat protein,the isolated wheat protein product may be separated from any residualbran remaining in the reactor. For example, the isolated wheat proteinproduct may be separated from the residual bran, or may be removed fromthe residual bran by other means known in the art.

In certain embodiments, the isolated wheat protein product from theagitation, such as the aqueous slurry of the isolated wheat protein, maybe held in a holding tank prior to drying. In certain embodiments, theholding tank may be equipped with a variable speed agitator, such as anagitator with a speed variable between 30 rpm and 500 rpm. Agitation ofthe slurry may aid in maintaining the isolated wheat protein in asoluble “liquid” form, such as a liquid having a viscosity ranging from20 cps to 1000 cps, and in another embodiment from 40 cps to 300 cps. Incertain embodiments, agitation of the isolated wheat protein product inthe holding tank may prevent the slurry from thickening, for example toa higher viscosity, while in the holding tank or may prevent formationof a film on the surface of the slurry. Maintaining the viscosity of theslurry at acceptable levels may also assist in transporting the slurryfrom one site to another, for example, when pumping the slurry from theholding tank to the dryer, such as via a transfer pipe or other transfermechanism used in the art.

Certain embodiments of the processes of the present disclosure mayfurther comprise drying the isolated wheat protein, for example, toremove water from the product. For example, the slurry comprising theisolated wheat protein that is substantially free of additives,processing aids, added acids, buffers, reagents, or added enzymes, maybe dried at an air temperature ranging from 320° F. to 500° F. Theisolated wheat protein may be dried for a time sufficient to provide anisolated wheat protein product having a product temperature ranging from160° F. to 230° F. In certain embodiments, the isolated wheat proteinmay be dried to a moisture content ranging from 0.1% to 10.0% moistureby weight. In other embodiments, the isolated wheat protein may be driedto a moisture content ranging from 0.1% to 7.0%; in still otherembodiments, a moisture content ranging from 4.0% to 6.0%; and in stillother embodiments, a moisture content of 5.0%. In still otherembodiments, the moisture content may be less than 7% by weight. Invarious embodiments, the internal temperature of the dryer may beincreased, wherein the higher temperature may damage the glutenstructure, thereby reducing its functionality (as shown by a lower linein a mixograph). The dried isolated wheat protein product may be cooledto room temperature. The isolated wheat protein may be dried by anymeans recognized in the art. For example, in one embodiment the isolatedwheat protein may be dried in a drying oven at a temperature from 320°F. to 500° F. The drying oven may be at ambient pressure or, in certainembodiments, the drying oven may be at reduced pressure. In otherembodiments, the isolated wheat protein may be dried by freeze drying,spray drying, ring drying, or flash drying. Various combinations of anyof these drying methods are also contemplated.

In various embodiments, the dried isolated wheat protein of the presentinvention may have a powder, granular, or particulate formulation. Forexample, according to one embodiment, the dried isolated wheat proteinmay be granular in form, having a size such that at least 85% of theisolated wheat protein grains are able to pass through a #60 mesh USBSsieve. That is, a #60 mesh sieve has sieve openings of 250 μM andtherefore, at least 85% of the isolated wheat protein grains will have asize less than 250 μM. In other embodiments, the dried isolated wheatprotein may have a granular form having a size such that at least 95% ofthe isolated wheat protein grains are able to pass through a #60 meshUSBS sieve.

In one embodiment, the wheat protein isolate of the present inventionmay have a solubility of between 62 and 99 in water. In anotherembodiment, the wheat protein isolate may have a mixograph of between 3and 10.

In other embodiments of the process for producing the isolated wheatprotein of the present invention, the isolated wheat protein may haveimproved characteristics as compared to a modified wheat protein isolatethat is not substantially free of additives, processing aids, addedacids, buffers, reagents, or added enzymes.

Improved foaming characteristics may include, for example, improved(i.e., greater) stiffness of foams, improved longevity or stability ofthe foam, and/or faster foaming time as compared to a modified wheatprotein isolate that is not substantially free of additives, processingaids, added acids, buffers, reagents, or added enzymes. For example,certain embodiments of the isolated wheat protein of the presentdisclosure may achieve a full foam in less time than it takes a modifiedwheat protein isolate that is not substantially free of additives,processing aids, added acids, buffers, reagents, or added enzymes toachieve a full foam. In other embodiments, the isolated wheat proteinproduced by the processes of the present disclosure may have a moredesirable flavor profile, such as, a blander flavor, than modified wheatprotein isolates that are not substantially free of additives,processing aids, added acids, buffers, reagents, or added enzymes.

In other embodiments, the processes of the present disclosure mayfurther comprise placing the isolated wheat protein into a container,such as a container that is configured for shipping. In still otherembodiments, the processes may further comprise associating indicia withthe container, wherein the indicia are capable of informing a user orconsumer of the content of the container. Still other embodiments mayfurther comprise shipping the container, for example by means of anairplane, ship, truck, railcar, or combinations of any of these shippingmethods. The container may be shipped, for example, from a firstgeographic location to a second geographic location.

Further embodiments of the present disclosure provide a process forproducing a food composition. The process may comprise incorporating anisolated wheat protein of the present invention into the foodcomposition. Food compositions may include, but are not limited to,baked goods, processed foods, whole grain foods, food bars, cereals,granolas, doughs and batters, cakes, tortillas, snacks, cookies,microwave bakery products, frozen foods, wieners, sausages, meat loaf,meat patties, whips, fillings, frostings, dressings, spreads, pet foods,fish feeds, shrimp feed, or animal feeds.

Other embodiments of the present disclosure provide a compositioncomprising an isolated wheat protein that may be free of additives,processing aids, added acids, buffers, reagents, or added enzymes. Stillother embodiments include a composition consisting essentially of anisolated wheat protein produced naturally, that is, without additives,processing aids, added acids, buffers, reagents, or added enzymes. Stillother embodiments include a composition consisting of an isolated wheatprotein produced naturally, that is, without additives, processing aids,added acids, buffers, reagents, or added enzymes.

Without intending to be limited by theory, it is thought that thenaturally produced wheat protein isolate of the present invention may beachieved by the natural production of organic acids during the reactionprocess. For instance, at the time and temperature of the reactionconditions, it is thought that microbes in the reaction vessel mayproduce natural, organic acids and/or other chemicals during growth ofthe microbes and that such organic acids and/or other chemicals may aidin the production of the natural wheat protein isolate of the presentinvention.

In other embodiments, the isolated wheat protein product may have a pHfrom 3.0 to 5.0. In certain embodiments, the pH of the isolated wheatprotein product may range from 3.9 to 4.9. The pH of the isolated wheatprotein may be adjusted to the desired pH level by the addition of oneor more basic or alkali ingredient or reagent, such as, for example,phosphate salts, soda ash, sodium bicarbonate, and other alkalireagents. Other embodiments of the compositions may further comprise aphosphate, such as a phosphate salt or phosphoric acid. One example of aphosphate salt that may be used includes, but is not limited to, sodiumphosphate. In one embodiment, the amount of phosphate that may be usedis a range of from 0.25-5% on a dry basis in the powder composition.Adjusting the pH of the isolated wheat protein product may result in anisolated wheat protein having one or more of good film formingproperties, good elastic properties, higher viscosity, or otherproperties.

EXAMPLES

The following exemplary, non-limiting examples are provided to furtherdescribe the embodiments presented herein. Those having ordinary skillin the art will appreciate that variations of these Examples arepossible within the scope of the invention.

Example 1

Wheat gluten was mixed with water to form a slurry for runs 1-13 at thesolids percent (%) of Table 1. The slurry was mixed at the temperaturesof Table 1 for each run for the amount of reaction time needed to reachabout 200 cps or between 170-240 cps, and the slurry was cut (under highshear mixing) every 15 minutes. The mixing was performed with anoverhead mixer for two hours to increase solubility. Throughout thereaction, pH, solubility, and viscosity were recorded. After thereaction, the slurry was over dried overnight and the dried product wasground. Tests were done to determine solubility, pH, and mixograph onthe final, ground product and the results are shown in Table 1. A graphof the mixograph is shown in FIG. 1, which shows a smooth lineindicative of a wheat protein isolate.

TABLE 1 Conditions used to produce the natural wheat protein isolate andproperties of natural wheat proteins produced according to Example 1.Tempera- Reaction time, ture, Solids Achieve Solu- Run ° C. % 200 cpbility pH Mixograph 1 36.0 15.50 380 67.2 4.00 9.2 2 34.6 13.75 380 64.24.04 9.7 3 39.5 13.75 245 66.7 4.05 9.8 4 44.4 13.75 200 74.3 3.96 7.9 539.5 13.75 225 65.2 4.10 8.3 6 43.0 15.50 210 68.9 4.04 7.7 7 39.5 13.75225 68.3 4.10 8.7 8 36.0 12.00 290 62.1 4.12 9.8 9 39.5 11.28 190 62.54.10 10 10 39.5 13.75 210 66.2 4.05 10 11 43.0 12.00 175 68.9 4.08 9.212 39.5 16.22 290 67.8 4.13 9.6 13 39.5 13.75 220 66.6 4.05 9.5

Example 2

6000 pounds of 30% solids wheat gluten was mixed with 520 gallons ofwater to form a slurry. The slurry was mixed at about 48.3° C. reactiontemperature for a 90 minute reaction time until the slurry reached about380 cps, at which point the slurry had a pH of about 4.2. The slurry wasspray dried to form the wheat protein isolate. A mixograph of the wheatprotein isolate produced in this example is shown in FIG. 2.

The wheat protein isolate of this example has a moisture content ofabout 4.5%; a protein of at least 90% (measured by nitrogen contenttimes 6.25); a pH of about 4.4; a mixograph of about 5.0; and a gravitysolubility of about 97%.

Example 3

520 gallons of water having a temperature of 122° F. and a pH of 6.6 wasplaced in a tank. 6000 pounds of 32.9% solids wheat gluten having a pHof 5.8 was added to the water and mixed with an agitator, thus forming aslurry. After about 25 minutes of mixing after the wheat gluten wasadded, the slurry had a pH of 4.9 and a temperature of 114° F. Afterabout 45 minutes of mixing after the gluten was added, the slurry had apH of 4.3, a temperature of 113° F., a % refractometer reading of 17.8%,and a Brookfield viscosity of 900 cps. After about 80 minutes of mixingafter the gluten was added, the slurry has a pH of 4.2, a %refractometer reading of 17.8%, and a Brookfield viscosity of 380 cps.At this time, the rate of mixing or agitation was slowed. After about 85minutes (i.e., a reaction time of 85 minutes) of mixing after the glutenwas added, the slurry had a pH of 4.2, a % refractometer reading of17.8%, and a Brookfield viscosity of 270 cps and the reaction wasstopped.

The slurry was sent to the decanter and spray dried. The final producthas the following characteristics: 93.8% protein (N×6.25) on a drybasis; a % solubility of 99.4% in a 10% solution in water; an excellentfoam test; and a mixograph of 6. A mixograph of the wheat proteinisolate produced in this example is shown in FIG. 3. An alveograph ofthe wheat protein isolate produced in this example is shown in FIG. 4.

This disclosure has been described with reference to certain exemplaryembodiments, compositions and uses thereof. However, it will berecognized by those of ordinary skill in the art that varioussubstitutions, modifications, or combinations of any of the exemplaryembodiments may be made without departing from the spirit and scope ofthe disclosure. Thus, the disclosure is not limited by the descriptionof the exemplary embodiments, but rather by the claims as originallyfiled.

1. A composition comprising: an isolated wheat protein; the compositionbeing substantially free of additives, processing aids, reagents, addedacids, buffers, and added enzymes; the composition having less than 10%moisture by weight.
 2. The composition of claim 1, wherein thecomposition has an additive, processing aids, reagents, added acids,buffers, and added enzymes concentration of less than 0.001% by weight.3. The composition of claim 1, wherein the composition has a proteincontent of at least 85% by weight as determined by multiplying anitrogen content of the composition by 6.25.
 4. The composition of claim1, wherein the isolated wheat protein is not enzyme hydrolyzed.
 5. Thecomposition of claim 1, wherein the composition has a granulation suchthat at least 85% of the composition is able to pass through a #60 USBSsieve.
 6. The composition of claim 1, being in a powder form.
 7. Thecomposition of claim 1, wherein the composition consists essentially ofthe isolated wheat protein produced naturally.
 8. The composition ofclaim 1, wherein the composition consists of the isolated wheat proteinproduced naturally.
 9. The composition of claim 1, the compositionhaving a protein content of at least 90% as determined by measuring thenitrogen content of the composition and multiplying by 6.25.
 10. Thecomposition of claim 1, wherein the composition has a solubility ofbetween 62 and 99 in water.
 11. The composition of claim 1, wherein thecomposition has pH of between 3.9 and 4.9.
 12. The composition of claim1, wherein the composition has a mixograph of between 3.0 and 10.0. 13.The composition of claim 1, further comprising a naturally producedenzyme.
 14. (canceled)
 15. A process for producing an isolated wheatprotein, comprising: mixing water and wheat gluten to form a glutenslurry; and agitating the gluten slurry at a temperature between 15° C.and 52° C. for a time sufficient to produce an isolated wheat protein,wherein an acid and butler are not added to the gluten slurry during theprocess.
 16. The process of claim 15, wherein the gluten slurry has anadditive, processing aid, reagent, added acid, buffer, and added enzymeconcentration of less than 0.001% by weight.
 17. The process of claim15, wherein the time ranges from 30 to 380 minutes.
 18. The process ofclaim 15, wherein the gluten slurry has a total solids content rangingfrom 6% to 22% on a dry basis.
 19. The process of claim 15, furthercomprising drying the isolated wheat protein.
 20. The process of claim15, wherein a viscosity of the gluten slurry reaches between 20-1000cps, thus producing the isolated wheat protein.
 21. The process of claim15, wherein a pH of the gluten slurry after mixing the water and thewheat gluten is above 4.5.
 22. (canceled)